Other parts of pineapple are also investigated for the presence of bromelain () including peel, core and crown etc. Extraction of bromelain from. EXTRACTION, PURIFICATION OF BROMELAIN FROM PINEAPPLE AND DETERMINATION OF ITS EFFECT ON BACTERIA CAUSING. et al recorded the first isolation of bromelain in from Bromelain is a crude protein extract obtained from pineapple fruit and stem, which comprises a.

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Purification and characterization of hydrolase with chitinase and chitosanase activity from commercial stem bromelain.

Anti-inflammatory agent Inflammation is the body’s attempt of self-protection which takes into account the discharge extractioon various factors that causes the distention of capillary and enhances their permeability, as a result of which the tissues appear to be inflated and inflamed Jaber Effects of oral bromelain administration on the impaired immunocytotoxicity of mononuclear cells from mammary tumor patients.

Bromelain is an efficient therapeutic agent and is being used successfully in allergic airway disease AAD Secor et al.

Effects of bromelain supplementation on growth performance, nutrient digestibility, blood profiles, faecal microbial shedding, faecal score and faecal noxious gas emission in weanling pigs. It possesses antithrombotic and anticoagulant activities and reduces platelet aggregation.

Bromelain: Methods of Extraction, Purification and Therapeutic Applications

Springer Science and Business Media V. Results of a dental study have showed that oral bromelain is an effective therapy to improve the quality of life after surgical removal of impacted lower third molars Tambourgi and Edgar Silveira: Liquid-liquid extraction of bromelain and polyphenol oxidase using aqueous two-phase system. Debriding effect of bromelain on firearm wounds in pigs.


Determination of optimum pH for enzyme activity: After treatment all the victims of PLC were fully recovered with zero ftom effects which is a virtue of its immuno-modulatory and antiviral trait Massimiliano et al.

Extraction of bromelain from pineapple peels.

Partitioning of bromelain from pineapple peel Nang Laecultv. Determination of the MIC: Modern strategies, such as membrane filtration Lopes et al. Characterization of Bromelain from Morindacitrifolia Noni. The MIC Minimum inhibitory concentration was determined. Article Information Sr No: Chen D, Huang S. Pineapple fruit bromelain affinity to different protein substrates. Bromelain treats asthma by decreasing the level of total BAL leukocytes eosinophils and lymphocytes and cellular infiltrates Secor et al.

Extraction of bromelain from pineapple peels.

In a study ethanolic extraction, isoelectric focusing and ammonium sulfate precipitation were compared for the extraction of bromelain. Efficacy of selected purification techniques bdomelain bromelain.

It is one of the extensively investigated proteolytic enzyme owing to its astonishing applications in various industries. Furthermore, bromelain promotes natural cell death apoptosis in cancerous cells Bhui et al. Bromelain has been effective in the treatment of cardio vascular diseases Tochi et al.

Application of an aqueous two-phase micellar system to extract bromelain from pineapple Ananascomosus peel waste and analysis of bromelain stability in cosmetic formulations.


J Exp Clin Cancer Res. Efficacy of reverse micellar extracted fruit bromelain in meat tenderization.

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Stover and Aline Dupont: Determination of the effects of the purified leaves and stembromelain on pathogens causing periodontitis. The minimum inhibitory concentration MIC of leaves and stem bromelain was found to be 0. Amongst these, ion exchange chromatography IEX is extensivelyemployed for bromelain separation Devi and Sowmiya, The reduction in collagen density is a common feature of periodontitis brpmelain is caused by the collagenase activity present often in bacteria Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis.

This is because the bromelain being a proteolytic enzyme breaks pineappple the peptide bonds in gelatin. Debridement refers to the elimination of dead, damaged, or infected tissue from the site of eschar to improve the healing potential of the remaining healthy tissues Rosenberg et al.

The commercially available bromelain is produced from pineapple stem but the extract is removed from cooled pineapple juice by centrifugation, ultra filtration and lyophilisation 3. How to cite this article: Akt a protein kinase controls numerous cellular processes and is a key player in cardiovascular diseases. Utilization of Pineapple Waste: